SciELO - Scientific Electronic Library Online

vol.84 issue1Allergic contact dermatitis to topical drugs: a descriptive analysisVitiligo and emotions author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Anais Brasileiros de Dermatologia

Print version ISSN 0365-0596On-line version ISSN 1806-4841


BRITO, Maila Karina Mattos de et al. Chitosan inclusions in the subcutaneous space of rats: clinic, histologic and morphometric evaluation. An. Bras. Dermatol. [online]. 2009, vol.84, n.1, pp.35-40. ISSN 0365-0596.

BACKGROUND: Chitosan is a polymer derivative from chitin applied in many medical specialties. OBJECTIVE:To evaluate the biocompatibility of chitosan membranes used as inclusion material into subcutaneous of rats. METHODS: Twenty male Wistar rats received chitosan membranes into the subcutaneous area of the dorsal medial region. The animals were randomly divided in four groups with 5 animals each, sacrificed on 7th (G1), 15th (G2), 30th (G3) and 60th (G4) postoperative day (PO). All animals were clinically evaluated daily and by photo-documentation at the sacrifice moment. The animals and the material were assessed for evidence of host response by histological and morphometric evaluation of the implants and the surrounding soft tissues. The material was fixed in 10% formalin and then the sections were stained with hematoxylin and eosin. RESULTS: None of animals presented side effects attributable to the implants. The histological evaluation showed smooth and homogeneous inclusions with no host cells inside and were encircled by a pseudocapsule of fibroblasts and inflammatory cells. The morphometric evaluation showed no statistical difference between different groups (P>0.05).

Keywords : Biocompatible materials; Chitosan; Materials testing; Rats, Wistar.

        · abstract in Portuguese     · text in English | Portuguese     · Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License