Nuclear morphometry and chromatin textural characteristics of basal cell carcinoma

Mendaçolli, Paola Jung; Brianezi, Gabrielli; Schmitt, Juliano Vilaverde; Marques, Mariângela Esther Alencar; Miot, Hélio Amante

doi:10.1590/abd1806-4841.20154076

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Dermatopathology • An. Bras. Dermatol. 90 (6) • Dec 2015 • https://doi.org/10.1590/abd1806-4841.20154076 copy

Nuclear morphometry and chromatin textural characteristics of basal cell carcinoma^* * Study performed at Departamento de Dermatologia e de Patologia da Faculdade de Medicina de Botucatu - Universidade Estadual Paulista "Júlio de Mesquita Filho" (Unesp) - Botucatu, SP, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract:

Histological subtypes of basal cell carcinoma have biological, evolutionary and distinct prognostic behavior. The analysis of characteristics of the nucleus can provide data on their cellular physiology and behavior. The authors of this study evaluated nuclear morphological parameters and textural patterns of chromatin from different subtypes of basal cell carcinoma: nodular (n=37), superficial (n=28) and sclerodermiform (n=28). The parameters were compared between neoplasms' subtypes and with unaffected adjacent basal epithelium. Nuclear area and diameter of sclerodermiform neoplasms were superior to the other subtypes. Chromatin's color intensity and fractal dimension were less intense in superficial subtypes. Nuclear roundness and chromatin's entropy presented lower values in tumors than in normal epithelium. There was significant correlation between morphological and textural variables of normal skin and tumors. Morphometric elements and textural chromatin's homogeneity of basal cell carcinomas may be related to evolutionary, biological and behavior particularities related to each histotype.

Keywords:
Carcinoma, basal cell; Chromatin; Karyometry; Image processing, computer-assisted; Prognosis

	Nodular (N=37)	Sclerodermiform (N=28)	Superficial (N=28)	Epithelium (N=93)
Area^* * micra2;	65.4 (23.1)	73.9 (29.6)	66.6 (21.8)	64.6 (17.6)
Perimeter^ micra	32.9 (7.7)	34.3 (7.9)	32.7 (6.1)	33.6 (5.9)
Circularity	0.77 (0.12)	0.78 (0.12)	0.78 (0.11)	0.73 (0.11)
Larger diameter^ micra	11.7 (2.7)	12.5 (2.9)	11.9 (2.5)	12.5 (2.2)
Intensity	62.7 (24.4)	61.5 (25.9)	54.8 (20.9)	43.5 (16.1)
Ra	236034.4 (7663.2)	233579.6 (12220.8)	234991.8 (8207.5)	234794.4 (5990.7)
Entropy	5.6 (0.51)	5.7 (0.58)	5.6 (0.55)	5.3 (0.53)
Fractal dimension	2.48 (0.05)	2.50 (0.05)	2.45 (0.05)	2.45 (0.05)

	Perimeter	Circularity	Larger diameter	Intensity	Ra	Entropy	Fractal
Area	0.87^* * p<0.01	0.12	0.76^* * p<0.01	0.43^* * p<0.01	0.90^* * p<0.01	0.23	0.70^* * p<0.01
Perimeter	-	0.55^* * p<0.01	0.90^* * p<0.01	0.47^* * p<0.01	0.73^* * p<0.01	0.03	0.03
Circularity	-	-	0.56^* * p<0.01	0.24	0.02	0.045^* * p<0.01	0.16
Larger diameter	-	-	-	0.39	0.65^* * p<0.01	0.12	0.47^* * p<0.01
Intensity	-	-	-	-	0.22	0.23	0.60^* * p<0.01
Ra	-	-	-	-	-	0.17	0.52^* * p<0.01
Entropy	-	-	-	-	-	-	0.10

	Perimeter	Circularity	Larger diameter	Intensity	Ra	Entropy	Fractal
Area	0.92^* * p<0.01	0.10	0.84^* * p<0.01	0.40^* * p<0.01	0.89^* * p<0.01	0.24	0.70^* * p<0.01
Perimeter	-	0.45^* * p<0.01	0.92^* * p<0.01	0.40^* * p<0.01	0.79^* * p<0.01	0.02	0.65*
Circularity	-	-	0.45^* * p<0.01	0.05	0.05	0.56^* * p<0.01	0.09
Larger diameter	-	-	-	0.28	0.74^* * p<0.01	0.08	0.48^* * p<0.01
Intensity	-	-	-	-	0.26	0.32	0.67^* * p<0.01
Ra	-	-	-	-	-	0.21	0.56^* * p<0.01
Entropy	-	-	-	-	-	-	0.24

	Tumor x Normal	Type of tumor	Observation
Area	3,30 (0,07)	5,33 (0,01)	Sclerodermiform higher than others
Perimeter	0,35 (0,55)	4,21 (0,02)
Circularity	41,23 (0,00)	3,57 (0,03)	Lower circularity in normal epithelium
Larger diameter	6,73 (0,01)	6,43 (0,00)	Sclerodermiform higher than others
Intensity	94,90 (0,00)	4,90 (0,01)	Superficial lower than others
Ra	0,49 (0,48)	3,44 (0,03)
Entropy	73,24 (0,00)	0,71 (0,49)	Lower entropy in normal epithelium
Fractal dimension	36,12 (0,00)	6,91 (0,01)	Superficial lower than others

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E-mail: revista@sbd.org.br

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[1] MAILING ADDRESS: Gabrielli Brianezi, Departamentos de Dermatologia e de Patologia Faculdade de Medicina de Botucatu - Unesp, Distrito de Rubião Jr. s/n, 18618-000 - Botucatu - SP, Brazil. Email: gabriellibrianezi@gmail.com

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Nuclear morphometry and chromatin textural characteristics of basal cell carcinoma* * Study performed at Departamento de Dermatologia e de Patologia da Faculdade de Medicina de Botucatu - Universidade Estadual Paulista "Júlio de Mesquita Filho" (Unesp) - Botucatu, SP, Brazil.

Abstract:

Nuclear morphometry and chromatin textural characteristics of basal cell carcinoma^* * Study performed at Departamento de Dermatologia e de Patologia da Faculdade de Medicina de Botucatu - Universidade Estadual Paulista "Júlio de Mesquita Filho" (Unesp) - Botucatu, SP, Brazil.