Print version ISSN 0366-6913
On-line version ISSN 1678-4553
FEDERMAN, S. R.; MANSUR, H. S.; STANCIOLI, E. F. B. and VASCONCELOS, W. L.. Biocompatibility evaluation of SiO2-CaO/stainless steel composite bioactive film for biomedical application. Cerâmica [online]. 2009, vol.55, n.335, pp.257-262. ISSN 0366-6913. http://dx.doi.org/10.1590/S0366-69132009000300004.
Sol-gel film in the SiO2-CaO system was prepared via reacting silicate precursor (TEOS - tetraethyl orthosilicate), calcium precursor (tetrahydrated calcium nitrate), alcohol in an acidic medium. After mixing, the coating was deposited on stainless steel by dip-coating technique at a low withdraw speed. After deposition, the composite was submitted to heat treatment, in air, at 400 ºC for 1 h. The composite biocompatibility has been analyzed by in vitro studies using two methods: a) concentrated simulated body fluid - SBF 1.5 - and b) cell culture. A scanning electron microscopy and energy dispersive spectrometer (SEM and EDS) analysis revealed the formation of hydroxyapatite precursor layer on bioactive film surface after 3 weeks into SBF solution. SEM analysis shows the adhesion, proliferation and spreading of cells on sol-gel derived bioactive film surface after 24 h of cellular culture, using VERO cells (ATCC CCL-81). Viability cellular essays with MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] indicated no citotoxicity in sol-gel film/VERO cells interface. Results suggested that this composite is an attractive material to be used in clinic field.
Keywords : coating; bioactive film; biocompatibility; SBF; cell culture.