SciELO - Scientific Electronic Library Online

 
vol.52 issue5Epidemiological profile of patients with extra-articular manifestations of rheumatoid arthritis from the city of Curitiba, South of BrazilNewly diagnosed dermatomyositis in the elderly as predictor of malignancy author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Revista Brasileira de Reumatologia

Print version ISSN 0482-5004

Abstract

KEUSSEYAN, Silene Peres et al. Diagnostic performance and clinical associations of antibodies to the chromatin antigenic system in juvenile systemic lupus erythematosus. Rev. Bras. Reumatol. [online]. 2012, vol.52, n.5, pp. 703-712. ISSN 0482-5004.  http://dx.doi.org/10.1590/S0482-50042012000500005.

OBJECTIVES: To determine the frequency of antibodies to chromatin components in juvenile systemic lupus erythematosus (JSLE), and to correlate the presence of these autoantibodies with clinical manifestations and disease activity. METHODS: Anti-chromatin (anti-CHR), anti-nucleosome core particle (anti-NCS) and anti-dsDNA antibodies were measured in 175 individuals, including 37 patients with active JSLE and 41 with inactive disease, 47 non-lupus autoimmune disease patients (non-lupus AD), and 50 healthy children. An in-house ELISA was developed with purified nucleosome core particles from calf thymus to determine IgG and IgG3 anti-NCS antibodies. Anti-CHR and anti-dsDNA antibodies were detected by commercial ELISA kits (INOVA). RESULTS: Anti-NCS and anti-CHR antibodies exhibited high specificity for JSLE and similar frequency in active and inactive JSLE. Anti-CHR and IgG/IgG3 anti-NCS serum levels did not differ between active and inactive JSLE. SLEDAI correlated with anti-dsDNA antibodies but not with antibodies to other chromatin components. There was association of anti-dsDNA, anti-CHR and IgG/IgG3 anti-NCS antibodies with proteinuria and low C4 serum levels. Anti-NCS antibodies in the absence of anti-dsDNA were observed in 14% of the JSLE patients. CONCLUSIONS: Our data indicate that anti-NCS and anti-CHR antibodies are relevant diagnostic markers for JSLE and appear to be correlated with JSLE lupus nephritis activity. IgG3 isotype anti-NCS antibodies do not seem to be more relevant than IgG anti-NCS antibodies as markers of disease activity or active nephritis in JSLE.

Keywords : nucleosomes; antinuclear antibodies; systemic lupus erythematosus; nephritis; chromatin.

        · abstract in Portuguese     · text in English | Portuguese     · pdf in English | Portuguese