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Ciência e Agrotecnologia

versão impressa ISSN 1413-7054

Resumo

MORAIS, Cecília Sandra Nunes et al. Effect of source and level of fat in the diet of wistar rats (rattus norvegicus) on lipoidal fractions in the serum. Ciênc. agrotec. [online]. 2003, vol.27, n.5, pp.1082-1088. ISSN 1413-7054.  https://doi.org/10.1590/S1413-70542003000500016.

Four fat sources (soybean oil, canola oil, olive oil, swine fat) were evaluated  with regard to their influence, at two levels in the diet (7 and 14%), on some fat fractions in rats' blood. Thus, eight groups of Wistar rat (Rattus norvegicus) males were ad libitum fed eight different diets, containing the fat and level reported, for 56 days. The dosages of total serum cholesterol and thriacylglicerols were performed by the colorimetric/enzymatic method utilizing a commercial specific kit. The HDL cholesterol was determined by precipitation of kilomicra (VLDL and LDL) by utilizing also a specific kit. The increase of fat in the diet to 14% raised the total cholesterol contents, serum HDL cholesterol and LDL + VLDL cholesterol when the source was swine fat. With 14% of fat in the diet, the lowest values of serum triacylglycerols were found for the fat sources soybean and canola oils, with values of 76.09 and 84.42mg/dl, respectively.

Palavras-chave : Fat sources; blood analyses; cholesterol; thriacylglicerols; rats.

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