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Ciência e Agrotecnologia
On-line version ISSN 1981-1829
RODRIGUES, Taislene Butarello et al. QTLs identification in common bean through SSR markers affected by natural selection. Ciênc. agrotec. [online]. 2007, vol.31, n.5, pp.1351-1357. ISSN 1981-1829. http://dx.doi.org/10.1590/S1413-70542007000500012.
Aiming to identify QTLs for grain yield and for 100 seed weight of common bean (Phaseolus vulgaris L.), microsatellite markers (SSR) affected by natural selection were selected in a population derived from the cross 'Carioca MG' x 'ESAL 686', advanced by the bulk breeding until F24. One hundred and seven F8 lines, and 107 F24, were evaluated in three environments: Winter of 2001 (F8:9 and F24:25) at Ijaci county; spring/summer of 2001 (F8:10 and F24:26) and summer/fall of 2002 (F8:11 and F24:27), both at Lavras county. Simple lattice 18 x 18 experimental design was used at Ijaci, and triple lattice in the others environments. Thirty polymorphic pair of primers were selected among 105, through the parents and a bulk of the F24 lines. The molecular and the experimental data were used in the multiple linear regression analysis (stepwise). Seven QTLs for grain yield were identified in F8 and six in F24, and the BM156 marker (derived from 'ESAL 686') showed the major effect. For 100 seed weight five QTLs were identified in F8 and two in F24, all of them derived from 'ESAL 686', and the major effect was exhibited by X61293 marker. Most of the QTLs expressed in only one environment.
Keywords : QTLs; microsatellite markers; Phaseolus vulgaris.