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Ciência e Agrotecnologia

versão impressa ISSN 1413-7054

Resumo

LEITZKE, Luciene Nolasco; DAMIANI, Claudia Roberta  e  SCHUCH, Márcia Wulff. The influence of culture medium, cytokinin type and concentrations on in vitro multiplication of blackberry and raspberry. Ciênc. agrotec. [online]. 2010, vol.34, n.2, pp.352-360. ISSN 1413-7054.  https://doi.org/10.1590/S1413-70542010000200012.

The blackberry and raspberry are species little cultivated in Brazil. However, they represent a valuable diversification option for small farms, once they are rustic and very productive. Root cuttings and softwood stem are the most widely practiced type of propagation. Recently, there has been an increased interest in the use of micropropagation as an alternative technique for fruit plant propagation. Nevertheless, there is a need to adjust the appropriate cultivation condition for each species and/or cultivar for the process to succeed. Therefore, this research aimed at determining the most suitable culture medium (MS and WPM), the effect of cytokinines (BAP, 2iP, Zea) and their concentrations (0; 7,5; 15 e 22,5μM) on in vitro multiplication of blackberry cultivar Xavante and raspberry cultivar Batum and Heritage. The results have shown that the medium MS and the cytokinin BAP at 13 μM for the blackberry 'Xavante' and 12 μM for raspberry 'Batum' and 'Heritage' were more efficient, increasing average leaf number, shoots and buds. For the blackberry, cultivation in MS medium in the absence of regulator promoted an increase in shoot length, whereas for 'Batum' and 'Heritage' raspberries, better results were obtained in the presence of citocinines - zeatin for Batum and 2iP for Heritage, independently of the concentrations.

Palavras-chave : Rubus idaeus; Rubus sp.; tissue culture; micropropagation; small fruits.

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