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Ciência e Agrotecnologia

versão impressa ISSN 1413-7054

Resumo

SOARES, Fernanda Pereira et al. Proliferation rate and residual effect of different sources of cytokinin on in vitro culture of Hancornia speciosa Gomes. Ciênc. agrotec. [online]. 2011, vol.35, n.1, pp.152-157. ISSN 1413-7054.  https://doi.org/10.1590/S1413-70542011000100019.

The mangabeira stands out for having a strong potential for fruit production. Its seeds present recalcitrance, making its propagation difficult, which makes much clear the need to obtain seedlings through asexual methods. In this context, the tissue culture presents as an alternative to be used. Among the factors that affect in vitro morphogenesis, the cytokinins should be highlighted as it influences the differentiation of buds and shoot growth. In this context the influence of three different sources of cytokinins (6-benzylaminopurine BAP, kinetin CIN and thidiazuron TDZ) was evaluated on the induction of in vitro mangabeira shoots, as well as the residual effect of this type of regulator on rooting and multiplication in successive subcultures. The results showed that among the tested cytokinins, the 6-benzylaminopurine was the one that induced a higher number of shoots (1.98), buds (19.22) and leaves (18.86) per explant, promoting the formation of shoots with higher length (4.55 cm). The shoots from basal WPM medium rooted easier. The 6-benzylaminopurine was responsible for the highest rate of multiplication (9.61) of mangabeira shoots. Sucessive subcultures decreased the capacity of shoot multiplication.

Palavras-chave : Micropropagation; mangabeira; subcultures.

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