SciELO - Scientific Electronic Library Online

 
vol.36 número2Diversidade genética em genótipos de Cedro Australiano selecionados via modelos mistosDeterminação da composição química e das propriedades funcionais de concentrado protéico e de farinha liofilizada de resíduos de camarão índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Ciência e Agrotecnologia

versão impressa ISSN 1413-7054

Resumo

NUNES, Renata de Castro; BUSTAMANTE, Fernanda de Oliveira; TECHIO, Vânia Helena  e  MITTELMANN, Andréa. Morphology and pollen viability of Lolium multiflorum Lam.. Ciênc. agrotec. [online]. 2012, vol.36, n.2, pp.180-188. ISSN 1413-7054.  https://doi.org/10.1590/S1413-70542012000200006.

Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum) were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P), equatorial diameter (E), exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM). All genotypes showed high rate of pollen viability (> 89%) for both stains. There were differences between genotypes in the following quantitative traits: polar axis, equatorial diameter, exine, endexine, ektexine, and P/E ratio. Pollen grains were characterized as small, monoporates, with circular and non-prominent apertures. In addition to helping distinction of pollen grains, morphometric differences can be used later to compare ploidy levels, thus assisting in breeding programs of the species.

Palavras-chave : Scanning electron microscopy; ryegrass; acetolysis.

        · resumo em Português     · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons