Acta Ortopédica Brasileira
Print version ISSN 1413-7852
MOROZ, Andrei et al. Platelet gel: 3D scaffold for cell culture. Acta ortop. bras. [online]. 2009, vol.17, n.2, pp. 43-45. ISSN 1413-7852. http://dx.doi.org/10.1590/S1413-78522009000200008.
INTRODUCTION: Tissue repair has been the ultimate goal of surgery. Cell culture requires a mechanical scaffold that supports cell growth and nutrient diffusion. Using platelet-rich plasma (PRP) as a 3D scaffold presents various advantages: it is a biological material, easily absorbed after transplantation, rich in growth factors, in particular, PDGF-ββ and TGF-β that stimulate extracellular matrix synthesis in cartilage culture. OBJECTIVE: To develop a PRP 3D scaffold. Material and METHODS: Two forms were idealized: Sphere and Carpet. Sterile conditions were used. The platelet gel remained in culture conditions, observed at an inverted microscope on a daily basis. RESULTS: Both forms were successful because they produced a 3D environment that supports cell growth, with positive and negative features. DISCUSSION: The Sphere form didn't attach to the plate. Gel retraction was observed and the investigation at the microscope was difficult, because of the opaque areas in the optical field. The Carpet form didn't retract, and didn't produce opaque areas. Follow-up time was 20 days. CONCLUSIONS: The production of a PRP 3D scaffold was successful, and this is an alternative requiring further investigation in order to establish an efficient and reliable route in tissue engineering technology, particularly in cartilage tissue culture.
Keywords : Cell culture techniques; Tissue scaffolds; Tissue engineering.