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Acta Ortopédica Brasileira

versão impressa ISSN 1413-7852


MARQUES, Fabiana Paulino et al. A manual method to obtain platelet rich plasma. Acta ortop. bras. [online]. 2014, vol.22, n.2, pp.75-77. ISSN 1413-7852.


This study is to report a manual method to obtain platelet rich plasma (PRP).


For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.


A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.


The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.

Palavras-chave : Platelet-rich plasma; Blood platelets; Methods..

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