Brazilian Journal of Infectious Diseases
versión impresa ISSN 1413-8670
DAUR, Alessandra Valle et al. Enrichment methodology to increase the positivity of cultures from body fluids. Braz J Infect Dis [online]. 2006, vol.10, n.6, pp. 372-373. ISSN 1413-8670. http://dx.doi.org/10.1590/S1413-86702006000600002.
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.
Palabras llave : Body fluids; etiological agents; enrichment.