SciELO - Scientific Electronic Library Online

vol.35 issue3The arrangements configured by the cranial and caudal mesenteric arteries in the domestic duck (Cairina moshata)Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Veterinary Research and Animal Science

Print version ISSN 1413-9596On-line version ISSN 1678-4456


MITSUKA, Regina et al. Toxoplasma gondii: II. Tachyzoite antigenic characterization of eigth strains. Braz. J. Vet. Res. Anim. Sci. [online]. 1998, vol.35, n.3, pp.00-00. ISSN 1413-9596.

Eight Toxoplasma gondii strains were analyzed using ELISA and Western blot techniques, in order to demonstrate possible immunological differences. The analyzed strains were: LIV IV, LIV V and S 11 isolated from swine, RH and VPS from a human being, AS 28 from a wild mouse, HV III from a dog and CN from a cat. With the ELISA assay the eight strains showed similar reactivity with homologous and heterologous sera. The antigenic suspension, consisting of total cellular extract of tachyzoites, was effective in the indirect ELISA assay, with the positive sera reacting strongly and the negative not reacting with the antigens. The Western blot analysis showed that the T. gondii strains have similar antigenic profiles with a few variations. Three bands were observed in all strains: one of about 33 kDa (p33), another of 54 kDa (p54) and a third one of 66 kDa (p66). The HV III strain, isolated from a dog, did not show three antigens (50, 70 and 75 kDa) that were present in the others. However, this difference was not detected by the ELISA assay. Only two antigens (62 kDa of the CN and 67 kDa of the LIV IV) were strain-specific antigens.

Keywords : Toxoplasma gondii; Antigens; Western blot; ELISA indirect test.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License