Brazilian Journal of Veterinary Research and Animal Science
On-line version ISSN 1678-4456
KORNDORFER, Clotilde Maria; MEIRELLES, Cyro Ferreira; BUENO, Ives Cláudio da Silva and ABDALLA, Adibe Luiz. Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay. Braz. J. Vet. Res. Anim. Sci. [online]. 1998, vol.35, n.3, pp. 00-00. ISSN 1678-4456. http://dx.doi.org/10.1590/S1413-95961998000300004.
The purpose of this study was to find a practical procedure for the extraction of progesterone (P4) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in the total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with 125I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (P<0.01).
Keywords : Progesterone; Feces; Radioimmunoassay; Rabbit.