Brazilian Journal of Veterinary Research and Animal Science
On-line version ISSN 1678-4456
ALVAREZ, Rafael Herrera et al. Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid. Braz. J. Vet. Res. Anim. Sci. [online]. 1998, vol.35, n.6, pp. 00-00. ISSN 1678-4456. http://dx.doi.org/10.1590/S1413-95961998000600007.
Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2a analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the experimental period and plasma concentrations of FSH, LH and progesterone were determined by radioimmunoassay. All cows from the immunized group, and 3 out of 5 cows in the control group, had more than 2 CL. There was no significant difference (P>0.05) in the ovulation rate between immunized and control groups (14.4 and 9.9, respectively). The number of recovered embryos was not significantly different (P>0.05) between groups, although cows immunized against bFF had a greater number of transferable embryos (3.4 ± 1.0 versus 0.8 ± 0.4, P<0.05). Plasma gonadotrophin concentrations were not correlated with either ovulation rate or number of recovered embryos. Plasma progesterone concentration was positively correlated (r = 0.88, P<0.01) to ovulation rate. Results suggested that passive immunization of cows with anti-bFF before superovulation did not reduce the variability of ovarian response.
Keywords : Superovulation; Follicular fluid; Passive immunization; Embryos; Cattle.