Genetics and Molecular Biology
On-line version ISSN 1678-4685
BARRIL, Nilce; CARVALHO-SALLES, Andréa Borduchi and TAJARA, Eloiza Helena. A rapid technique for analysis of formalin-fixed, paraffin-embedded tissues by fluorescent in situ hybridization with alpha-satellite probes. Genet. Mol. Biol. [online]. 1998, vol.21, n.4, pp.-. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47571998000400006.
We describe a rapid procedure for preparing archival tissues for interphase FISH analysis. The present protocol differs from others previously described because it allows the obtention of nuclei in satisfactory number and quality without using special equipments, adhesive-treated slides or solutions for chromatin decondensation. The method is of low cost and useful for retrospective analyses of formalin-fixed, paraffin-embedded samples.