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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685


LEAL, Tereza Cristina A.; LEAL, Nilma C.  and  ALMEIDA, Alzira M. Paiva de. RAPD-PCR typing of  Yersinia enterocolitica (Enterobacteriaceae) O:3 serotype strains isolated from pigs and humans. Genet. Mol. Biol. [online]. 1999, vol.22, n.3, pp.315-319. ISSN 1415-4757.

Sixteen strains of Yersinia enterocolitica serotype O:3, isolated from apparently healthy pigs collected in Rio de Janeiro, and four human strains of serotypes O:4, O:5, O:6 and O:13 were analyzed by RAPD-PCR. The strains were grouped into five genotypic profiles according to the amplification patterns obtained with three random primers. Fifteen of the 16 pig strains had identical amplification patterns, which was named genotypic profile 1. The one different profile was named genotypic profile 2. Genotypic profile 1 was also exhibited by the O:6 human serotype strain. The O:4 and O:13 human serotype strains showed similar amplification profiles with two primers. However, the third primer induced a distinct profile in each strain. Therefore, these two strains were placed into genotypic profile 3 and 4, respectively. Each primer produced a completely different amplification profile in the O:5 human serotype strain; therefore, it was named genotypic profile 5. The presence or absence of plasmids in the strains studied did not affect the amplification results. These results show that genetic variations can exist within a serotype, and strains of different serotypes can exhibit the same amplification profile when compared using other primers.

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