SciELO - Scientific Electronic Library Online

 
vol.22 issue3Varroa jacobsoni infestation of adult Africanized and Italian honey bees (Apis mellifera) in mixed colonies in BrazilAutosomal rearrangement in Gryllus assimilis Fabricius, 1775 (Orthoptera, Gryllidae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

SANTOS, Joselita Maria Mendes dos; LOBO, Jorge Arturo; TADEI, Wanderli Pedro  and  CONTEL, Eucleia P. Betioli. Intrapopulational genetic differentiation in Anopheles (N.) darlingi Root, 1926 (Diptera: Culicidae) in the amazon region . Genet. Mol. Biol. [online]. 1999, vol.22, n.3, pp. 325-331. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47571999000300007.

Four natural Amazonian Anopheles darlingi populations were analyzed for 19 loci, 12 of which showed variation, i.e., 68.4% of polymorphic loci. The Ariquemes, Rondônia, population was the most variable, with a large number of alleles per locus (2.26 ± 0.27) and high polymorphism (P = 63.15). The highest values of observed and expected intralocus heterozygosity were observed in the Manaus, Amazonas, population (Ho = 0.432 ± 0.11; He = 0.375 ± 0.08), and the lowest in the Cachoeira Porteira, Pará, population (Ho = 0.236 ± 0.09; He = 0.290 ± 0.11). Wright's F-statistic revealed disequilibrium caused by an excess of homozygotes, as shown by the Fis > Fst values (Fis = 0.083 > 0.026) reflecting intrapopulational differentiation. The four populations studied were genetically similar, as indicated by distance and similarity values. Chromosomal variation of Amazon population also did not indicate geographical differentiation, and populations in the central region of the Amazon Basin showed high polymorphism in relation to the marginal populations, which were mainly monomorphic.

        · abstract in Portuguese     · text in English     · pdf in English