Genetics and Molecular Biology
On-line version ISSN 1678-4685
DUARTE, José Maurício Barbanti; RAMALHO, Mariney Flávia Pereira Di Tano; LIMA, Vera Fernanda Hossepian de and JORGE, Wilham. A leukocyte cryopreservation technique for cytogenetic studies . Genet. Mol. Biol. [online]. 1999, vol.22, n.3, pp. 399-400. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47571999000300019.
Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.