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Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

DUARTE, José Maurício Barbanti; RAMALHO, Mariney Flávia Pereira Di Tano; LIMA, Vera Fernanda Hossepian de  and  JORGE, Wilham. A leukocyte cryopreservation technique for cytogenetic studies . Genet. Mol. Biol. [online]. 1999, vol.22, n.3, pp. 399-400. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47571999000300019.

Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.

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