Genetics and Molecular Biology
Print version ISSN 1415-4757
KEMENES, Paola Augusta et al. k-Casein, b-lactoglobulin and growth hormone allele frequencies and genetic distances in Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis cattle. Genet. Mol. Biol. [online]. 1999, vol.22, n.4, pp. 539-541. ISSN 1415-4757. http://dx.doi.org/10.1590/S1415-47571999000400012.
The genotypes for k-casein (k-CN), b-lactoglobulin (b-LG) and growth hormone (GH) were determined by polymerase chain reaction (PCR) and restriction enzyme digestion in seven breeds of cattle (Nelore, Gyr, Guzerá, Caracu, Charolais, Canchim and Santa Gertrudis). k-Casein had two alleles with the A allele occurring at a higher frequency in Bos indicus breeds (0.93, 0.92 and 0.91% for Gyr, Guzerá and Nelore, respectively). The b-lactoglobulin locus had two alleles in all of the breeds. European breeds had a higher frequency of the b-LG A allele than Zebu breeds. The GH locus had two alleles (L and V) in Bos taurus and was monomorphic (L allele only) in all of the Bos indicus breeds evaluated. The highest frequency for the V allele was observed in Charolais cattle. The markers used revealed a considerable similarity among breeds, with two main groups being discernible. One group consisted of Zebu and Santa Gertrudis breeds and the other consisted of European and Canchim breeds.