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Genetics and Molecular Biology
On-line version ISSN 1678-4685
TOMASCO, Ivanna; WLASIUK, G. and LESSA, E.P.. Evaluation of polymorphism in ten microsatellite loci in Uruguayan sheep flocks. Genet. Mol. Biol. [online]. 2002, vol.25, n.1, pp.37-41. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47572002000100008.
The allele frequencies of 10 microsatellite loci previously described for sheep as BM1314, BM6526, OarFCB128, OarHH64, OarCP20, OarHH47, OarFCB48, OarHH35, OarHH72 and BM2508 were estimated for the Uruguayan flocks. A representative sample of 101 individuals composed by the two predominant breeds (76% Corriedale and 24% Australian Merino) was used. The sample did not show a significant tendency towards substructuring, in spite of presenting some significantly different allele frequencies between races. The Corriedale sample presents three loci in which the presence of null alleles is possible. The markers were highly variable, showing between 7 and 15 alleles each. The Polymorphism Information Content Index ranged from 0.63 to 0.87 and the Exclusion Probability from 0.39 to 0.75 for a cumulative Exclusion Probability of 99.98%. These results suggest the effectiveness of this set of loci for testing genetic relatedness. This is the first report of microsatellite variation in Corriedale.
Keywords : sheep; polymorphism; microsatellites.