SciELO - Scientific Electronic Library Online

vol.25 número2Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopyMorphological characterization and genetic analysis of Drechslera teres isolates índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Genetics and Molecular Biology

versão impressa ISSN 1415-4757versão On-line ISSN 1678-4685


PINTO, Fabiana Gisele da Silva et al. Genetic variation in the cuticle-degrading protease activity of the entomopathogen Metarhizium flavoviride. Genet. Mol. Biol. [online]. 2002, vol.25, n.2, pp.231-234. ISSN 1415-4757.

Extracellular proteases have been shown to be factors of virulence in fungal entomopathogenicity. We examined the production of the cuticle-degrading extracellular proteases chymoelastase (Pr1) and trypsin (Pr2) in isolates of the fungus Metarhizium flavoviride. Fungal growth was in a mineral medium (MM) containing nitrate, and in MM supplemented with either cuticle from Rhammatocerus schistocercoides or with the non-cuticular substrate casein. The substrates used for growth influenced the expression of both analyzed proteases, the highest protease activities of nearly all isolates having been observed in the medium containing insect cuticle, with more Pr1 than Pr2 being produced. There was a natural variability in the production of cuticle-degrading proteases among isolates, although this was less evident for Pr2. Our data support the hypothesis that the production of Pr1 on insect cuticle is a useful characteristic for the analysis of intraspecific variability of M. flavoviride isolates.

Palavras-chave : Metarhizium flavoviride; Rhammatocerus schistocercoides; chymoelastase (subtilisin) (Pr1); trypsin (Pr2).

        · texto em Inglês     · Inglês ( pdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons