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Genetics and Molecular Biology
On-line version ISSN 1678-4685
DOMINGUES, Alayne Magalhães Trindade et al. Karyotype characterization of Trigona fulviventris Guérin, 1835 (Hymenoptera, Meliponini) by C banding and fluorochrome staining: report of a new chromosome number in the genus. Genet. Mol. Biol. [online]. 2005, vol.28, n.3, pp. 390-393. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47572005000300009.
Although many species of the genus Trigona have been taxonomically described, cytogenetic studies of these species are still rare. The aim of the present study was to obtain cytogenetic data by conventional staining, C banding and fluorochrome staining for the karyotype characterization of the species Trigona fulviventris. Cytogenetic analysis revealed that this species possesses a diploid chromosome number of 2n = 32, different from most other species of this genus studied so far. This variation was probably due to the centric fusion in a higher numbered ancestral karyotype, this fusion producing the large metacentric chromosome pair and the lower chromosome number observed in Trigona fulviventris. Heterochromatin was detected in the pericentromeric region of the first chromosome pair and in one of the arms of the remaining pairs. Base-specific fluorochrome staining with 4'-6-diamidino-2-phenylindole (DAPI) showed that the heterochromatin was rich in AT base pairs (DAPI+) except for pair 13, which was chromomycin A3 (CMA3) positive indicating an excess of GC base pairs. Our data also suggests that there was variation in heterochromatin base composition.
Keywords : cytogenetics; heterochromatin; evolution; stingless bees.