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Genetics and Molecular Biology
On-line version ISSN 1678-4685
YU, Kangfu et al. Absence of the A4 peptide in the G4 glycinin subunit of soybean cultivar Enrei is caused by a point mutation in the Gy4 gene. Genet. Mol. Biol. [online]. 2005, vol.28, n.3, pp. 440-443. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47572005000300018.
Functional properties of soy proteins for food are closely related to the composition of their storage protein subunits. Using base excision sequence scanning (BESS), we show that the absence of the A4 peptide in the G4 glycinin subunit of the soybean (Glycine max L.) cultivar Enrei was caused by the same point mutation in the Gy4 gene as previously reported in the soybean cultivar Raiden. Although the genetic relationship between Raiden and Enrei is not known, the same point mutation in their Gy4 genes may indicate that they probably share a related origin. The application of BESS to identify single nucleotide polymorphisms (SNPs) as co-dominant markers for marker-assisted selection (MAS) of a recessive null allele is also discussed.
Keywords : base excision sequence scanning (BESS); marker-assisted selection (MAS); single nucleotide polymorphisms (SNPs); seed storage protein; soybean.