SciELO - Scientific Electronic Library Online

 
vol.28 issue3Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresisGenotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

SILVA-BARBOSA, Isvânia et al. Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation . Genet. Mol. Biol. [online]. 2005, vol.28, n.3, pp. 452-457. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572005000300021.

Scoring of unstable chromosome aberrations (dicentrics, rings and fragments) and micronuclei in circulating lymphocytes are the most extensively studied biological means for estimating individual exposure to ionizing radiation (IR), which can be used as complementary methods to physical dosimetry or when the latter cannot be performed. In this work, the quantification of the frequencies of chromosome aberrations and micronuclei were carried out based on cytogenetic analyses of peripheral blood samples from 5 patients with cervical uterine cancer following radiotherapy in order to evaluate the absorbed dose as a result of partial-body exposure to 60Co source. Blood samples were collected from each patient in three phases of the treatment: before irradiation, 24 h after receiving 0.08 Gy and 1.8 Gy, respectively. The results presented in this report emphasize biological dosimetry, employing the quantification of chromosome aberrations and micronuclei in lymphocytes from peripheral blood, as an important methodology of dose assessment for either whole or partial-body exposure to IR.

Keywords : chromosome aberrations; micronuclei; biodosimetry; partial-body irradiation; lymphocyte.

        · text in English     · pdf in English