Abstract

CAGLIARI, Thiago C.; TIROLI, Ana O.; BORGES, Júlio C.  and  RAMOS, Carlos H.I.. Identification and in silico expression pattern analysis of Eucalyptus expressed sequencing tags (ESTs) encoding molecular chaperones. Genet. Mol. Biol. [online]. 2005, vol.28, n.3, suppl., pp. 520-528. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572005000400006.

Expressed Sequence Tags (ESTs) sequencing provides reliable and useful information concerning gene expression patterns in the genomic context. Our group used bioinformatics to identify and annotate 5'EST-contigs belonging to the molecular chaperones within the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We found that 1,959 5'EST-contigs, or approximately 1.6% of the total 5'EST-contigs, encoded chaperones, emphasizing their biological importance. About 55% of the chaperones that we found were Hsp70 chaperones and its co-chaperones, 18% were Hsp90 chaperones, 15% were Hsp60 and its co-chaperone, 8% were Hsp100 chaperones, and 4% were Small Hsps. We also investigated the digital expression profile of the chaperone genes to gain information on gene expression levels in the different libraries and we found that molecular chaperones may have differential expression. The results discussed here give important hints about the role of chaperones in Eucalyptus cells.

Keywords : chaperones; heat shock proteins; genome; expressed sequence tags.