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Genetics and Molecular Biology
On-line version ISSN 1678-4685
BHUSHAN, Bharat et al. Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene. Genet. Mol. Biol. [online]. 2007, vol.30, n.3, pp. 560-566. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47572007000400009.
The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.
Keywords : MHC; BoLa-A polymorphism; cattle.