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Genetics and Molecular Biology
On-line version ISSN 1678-4685
LUCCA JR., Marcos de; CARARETO, Claudia Marcia Aparecida and CERON, Carlos Roberto. Distribution of the Bari-I transposable element in stable hybrid strains between Drosophila melanogaster and Drosophila simulans and in Brazilian populations of these species. Genet. Mol. Biol. [online]. 2007, vol.30, n.3, pp.676-680. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47572007000400028.
We analyzed the distribution of the Bari-I transposable element in Drosophila melanogaster (IN(1)AB), its sibling species Drosophila simulans (C167.4) and in eight hybrid strains derived from initial crosses involving D. simulans females and D. melanogaster males of the above cited strains as well as in Brazilian populations of these species. Polymerase chain reaction (PCR) data showed the presence of the Bari-I element among species populations and hybrid strains. Hybridization with a 703 bp probe homologous to the Bari-I sequence showed that the number of Bari-I copies in D. melanogaster IN(1)AB was higher than in D. simulans C167.4 strains. Hybrid strains presented Bari-I sequences related to both parental species. In addition some strains displayed a Bari-I sequence that came from D. melanogaster, suggesting introgression of D. melanogaster genetic material in the background of D. simulans. In contrast, some hybrids showed deletions of D. simulans Bari-I sequences.
Keywords : Bari-I; Drosophila melanogaster; D. simulans; introgressive hybridization; transposable element.