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vol.30 issue3  suppl.In silico analysis of phytohormone metabolism and communication pathways in citrus transcriptomeIn silico prediction of gene expression patterns in Citrus flavedo author indexsubject indexarticles search
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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685

Abstract

MEHTA, Angela et al. Signaling pathways in a Citrus EST database. Genet. Mol. Biol. [online]. 2007, vol.30, n.3, suppl., pp.734-751. ISSN 1415-4757.  https://doi.org/10.1590/S1415-47572007000500003.

Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis) was launched in order to sequence Citrus ESTs (expressed sequence tags) from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile). Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

Keywords : Citrus; cell signaling; genomics.

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