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vol.30 issue3  suppl.Citrus plastid-related gene profiling based on expressed sequence tag analysesIdentification of protein kinase SNF1 in CitEST author indexsubject indexarticles search
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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685


AMARAL, Alexandre Morais do et al. Identification of citrus expressed sequence tags (ESTs) encoding pleiotropic drug resistance (PDR)-like proteins. Genet. Mol. Biol. [online]. 2007, vol.30, n.3, suppl., pp.857-865. ISSN 1415-4757.

Pleiotropic drug resistance (PDR) proteins, a subfamily of the ATP-binding cassette (ABC) transporters, have been recently shown to play a role in plant defense against biotic and abiotic stresses. However, nothing is known about their expression in citrus. To investigate the occurrence of PDR homologues in citrus species, we have surveyed EST sequences from different tissues and conditions of the Citrus Expressed Sequence Tags (CitEST) database, through sequence similarity search analyses and inspections for characteristic PDR domains. Multiple sequence alignments, prediction of transmembrane topology and phylogenetic analysis of PDR-like proteins were additionally performed. This study allowed the identification of nine putative proteins showing characteristic PDR features in citrus species under various conditions, which may indicate a potential correlation between PDRs and stress and metabolism of citrus plants. Moreover, a tissue-specific putative PDR-like protein was found in sweet orange fruits. To our knowledge, this is the first report regarding the identification of citrus ESTs encoding PDR-like proteins as well as the first to identify a putative full ABC transporter with specific expression in fruits.

Keywords : ABC transporters; CitEST; expressed sequence tag; transcriptome; stress.

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