Genetics and Molecular Biology
On-line version ISSN 1678-4685
LOPES, Denilce Meneses; POMPOLO, Sílvia das Graças; CAMPOS, Lúcio Antônio de Oliveira and TAVARES, Mara Garcia. Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining. Genet. Mol. Biol. [online]. 2008, vol.31, n.1, pp. 49-52. ISSN 1678-4685. http://dx.doi.org/10.1590/S1415-47572008000100010.
The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The euchromatin was restricted to the chromosome extremities and was GC-rich, as evidenced by CMA3 staining. The B chromosome was CMA3-negative and DAPI-positive, a heterochromatic constitution similar to that of the A genome chromosomes.
Keywords : Melipona; stingless bees; B chromosomes; fluorochromes; C-banding.