Genetics and Molecular Biology
Print version ISSN 1415-4757
On-line version ISSN 1678-4685
FRANCO, Fernando F.; SENE, Fabio M. and MANFRIN, Maura H.. Molecular characterization of SSS139, a new satellite DNA family in sibling species of the Drosophila buzzatii cluster. Genet. Mol. Biol. [online]. 2008, vol.31, n.1, pp.155-159. ISSN 1415-4757. http://dx.doi.org/10.1590/S1415-47572008000100026.
We characterized sequences of a novel SSS139 RsaI satellite DNA family in Drosophila gouveai and Drosophila seriema, two members of the Drosophila buzzatii cluster (D. repleta group). The sequences were AT-rich (69%) with a monomer unit length of about 139 bp and contained two direct subrepeats of 14 bp and 16 bp, suggesting that it might have originated by the duplication of smaller sequences. Southern and dot-blot hybridization analyses also detected SSS139 in other Drosophila buzzatii cluster species (D. koepferae, D. antonietae, D. borborema and D. serido) but not in D. buzzatii. These results agree with the marginal phylogenetic position of D. buzzatii within the D. buzzatii cluster.
Keywords : Drosophila buzzatii cluster; molecular evolution; repetitive DNA; satellite DNA; SSS139 satellite DNA.