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vol.31 issue2Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)The mitochondrial genome of the stingless bee Melipona bicolor (Hymenoptera, Apidae, Meliponini): sequence, gene organization and a unique tRNA translocation event conserved across the tribe Meliponini author indexsubject indexarticles search
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Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685

Abstract

SOUZA, Rogério O.; MORETTO, Geraldo; ARIAS, Maria C.  and  DEL LAMA, Marco A.. Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns. Genet. Mol. Biol. [online]. 2008, vol.31, n.2, pp.445-450. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572008000300009.

Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M. quadrifasciata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.

Keywords : subspecies differentiation; Melipona quadrifasciata; cytochrome b; mtDNA; PCR-RFLP patterns.

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