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Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

SINGH, Ram K. et al. SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity. Genet. Mol. Biol. [online]. 2010, vol.33, n.2, pp.319-324.  Epub Mar 26, 2010. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572010005000024.

Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly moderate bootstrap support. These major clusters corresponded with the photoperiod response and place of origin. The results indicate that the photoperiod insensitive genotypes, 11/2/1939 (EC 325097) and MACS 330 would be better choice for broadening the genetic base of soybean for this trait.

Keywords : photoperiod response; SSR; AFLP; genetic diversity; soybean.

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