Abstract

CHEN, Congying et al. Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene. Genet. Mol. Biol. [online]. 2010, vol.33, n.3, pp. 471-474.  Epub June 25, 2010. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572010005000057.

Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.

Keywords : expression profile; molecular characterization; physical mapping.