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Genetics and Molecular Biology
Print version ISSN 1415-4757
TAVARES, Mara Garcia; CARVALHO, Carlos Roberto; SOARES, Fernanda Aparecida Ferrari and FERNANDES, Anderson. Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae). Genet. Mol. Biol. [online]. 2010, vol.33, n.3, pp. 491-493. ISSN 1415-4757. http://dx.doi.org/10.1590/S1415-47572010000300019.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Keywords : cytogenetic; flow cytometry; genome size; karyotype; stingless bee.