Genetics and Molecular Biology
Print version ISSN 1415-4757
ZHAO, Yu; ZHANG, Shi-Bao; YANG, Jing and ZHANG, Lu. Characterization of 13 microsatellite loci developed from Meconopsis horridula. Genet. Mol. Biol. [online]. 2010, vol.33, n.3, pp. 539-541. Epub July 09, 2010. ISSN 1415-4757. http://dx.doi.org/10.1590/S1415-47572010005000066.
Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.
Keywords : genetic structure; heterozygosity; Meconopsis horridula; microsatellite markers; polymorphism.