Genetics and Molecular Biology
Print version ISSN 1415-4757
JAMSARI, Amirul Firdaus Jamaluddin; MIN-PAU, Tan and SITI-AZIZAH, Mohd Nor. Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata. Genet. Mol. Biol. [online]. 2011, vol.34, n.2, pp.345-347. Epub Apr 01, 2011. ISSN 1415-4757. http://dx.doi.org/10.1590/S1415-47572011005000007.
Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
Keywords : Channa striata; population genetics; microsatellite; multiplex PCR.