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Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

ALTROCK, Sarah; FONSECA, Artur  and  PEDROSA-HARAND, Andrea. Chromosome identification in the Andean common bean accession G19833 (Phaseolus vulgaris L., Fabaceae). Genet. Mol. Biol. [online]. 2011, vol.34, n.3, pp.459-463.  Epub July 29, 2011. ISSN 1415-4757.  https://doi.org/10.1590/S1415-47572011005000029.

Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA) were used as probes. Comparison to the Mesoamerican accession BAT93 showed little divergence, except for additional 45S rDNA sites in four chromosome pairs. Altogether, the results indicated a relative karyotypic stability during the evolution of the Andean and Mesoamerican gene pools of P. vulgaris.

Keywords : common bean; repetitive DNA; chromosome marker; cytogenetic map; comparative map; intraspecific variation.

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