Genetics and Molecular Biology
versão impressa ISSN 1415-4757
LOPEZ, Molkary Andrea; STASHENKO, Elena E. e FUENTES, Jorge Luis. Chemical composition and antigenotoxic properties of Lippia alba essential oils. Genet. Mol. Biol. [online]. 2011, vol.34, n.3, pp. 479-488. Epub 29-Jul-2011. ISSN 1415-4757. http://dx.doi.org/10.1590/S1415-47572011005000030.
The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs) from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS) analysis. The major compounds encountered being citral (33% geranial and 25% neral), geraniol (7%) and trans-β-caryophyllene (7%) for L. alba specimen COL512077, and carvone (38%), limonene (33%) and bicyclosesquiphellandrene (8%) for the other, COL512078. The genotoxicity and antigenotoxicity of EO and the compounds citral, carvone and limonene, were assayed using the SOS Chromotest in Escherichia coli. The EOs were not genotoxic in the SOS chromotest, but one of the major compound (limonene) showed genotoxicity at doses between 97 and 1549 mM. Both EOs protected bacterial cells against bleomycin-induced genotoxicity. Antigenotoxicity in the two L. alba chemotypes was related to the major compounds, citral and carvone, respectively. The results were discussed in relation to the chemopreventive potential of L. alba EOs and its major compounds.
Palavras-chave : Lippia alba; essential oil; antigenotoxicity; bleomycin; SOS chromotest.