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Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

COELHO, Mariza M.; FERREIRA-NOZAWA, Monica S.; NOZAWA, Sérgio R.  and  SANTOS, André L.W.. Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene. Genet. Mol. Biol. [online]. 2011, vol.34, n.4, pp. 676-680.  Epub Sep 16, 2011. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572011005000045.

Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species.

Keywords : isolation; sequencing; Bacillus; Pantoea.

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