Resumo

NASKAR, Soumen et al. Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis). Genet. Mol. Biol. [online]. 2012, vol.35, n.1, pp. 95-98.  Epub 02-Fev-2012. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572012005000013.

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β₁ domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.

Palavras-chave : water buffalo; cDNA; DRB; MHC; phylogeny.