SciELO - Scientific Electronic Library Online

 
vol.35 número1The cytotoxic and growth inhibitory effects of palladium(II) complexes on MDA-MB-435 cellsCloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine) índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

Links relacionados

Compartilhar


Genetics and Molecular Biology

versão impressa ISSN 1415-4757

Resumo

DU, Yue-feng et al. Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1. Genet. Mol. Biol. [online]. 2012, vol.35, n.1, pp. 164-171.  Epub 16-Fev-2012. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572012005000021.

Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.

Palavras-chave : prostate cancer; DNA methylation; DNMT3; RNA interference.

        · texto em Inglês     · pdf em Inglês