SciELO - Scientific Electronic Library Online

vol.35 issue1  suppl.1Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a reviewSubtractive libraries for prospecting differentially expressed genes in the soybean under water deficit author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Genetics and Molecular Biology

Print version ISSN 1415-4757


MOLINA, Lorrayne Gomes et al. Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries. Genet. Mol. Biol. [online]. 2012, vol.35, n.1, suppl.1, pp.292-303. ISSN 1415-4757.

A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantations and samples from soybean cultivated in greenhouses under a controlled environment were made. Different pathogenic, symbiotic and free-living organisms were identified from samples of both growth systems. They included viruses, bacteria and different groups of fungi. This approach can be useful not only to identify potentially unknown pathogens and pests, but also to understand the relations that soybean plants establish with microorganisms that may affect, directly or indirectly, plant health and crop production.

Keywords : next generation sequencing; small RNA; siRNA; molecular markers.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License