SciELO - Scientific Electronic Library Online

 
vol.35 issue1  suppl.1Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybeanMethod optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google
  • uBio

Share


Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

RODRIGUES, Elisete Pains et al. A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots. Genet. Mol. Biol. [online]. 2012, vol.35, n.1, suppl.1, pp. 348-352. ISSN 1415-4757.  http://dx.doi.org/10.1590/S1415-47572012000200016.

Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made in three replicates using both the original and simplified procedure. To evaluate the quality of the extracted proteins, ten spots were randomly selected and identified by mass spectrometry (MS). The 2-DE gels were equally well resolved, with no streaks or smears, and no significant differences were observed in protein yield, reproducibility, resolution or number of spots. Mass spectra of the ten selected spots were compared with database entries and allowed high-quality identification of proteins. The simplified protocol described here presents considerable savings of time and reagents without compromising the quality of 2-DE protein profiles and compatibility with MS analysis, and may facilitate the progress of proteomics studies of legume-rhizobia interactions.

Keywords : 2-DE; protein extraction; proteomics; soybean roots; symbiosis.

        · text in English     · English (pdf) English (epdf)