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Genetics and Molecular Biology

Print version ISSN 1415-4757

Abstract

ZHANG, Shumin et al. Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts. Genet. Mol. Biol. [online]. 2012, vol.35, n.3, pp.680-685.  Epub July 05, 2012. ISSN 1415-4757.  https://doi.org/10.1590/S1415-47572012005000042.

β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediating stable shRNA interference in goat-fetal fibroblast cells. Apart from high efficiency in the knockdown of BLG expression in these cells, lentivector-mediated RNAi manifested stable integration into the goat genome itself. Consequently, an in vitro model for goat BLG-content control was compiled, and a goat-cell line for accompanying transgenetic goat production created.

Keywords : RNAi; shRNA; lentivirus; BLG; goat.

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