Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of <i>Oryza sativa</i> L. and <i>Phaseolus vulgaris</i> L. from gene banks

Dantas, Alisson F.; Lopes, Renata M.; Fascineli, Maria L.; José, Solange C.B.R.; Pádua, Juliano G.; Gimenes, Marcos A.; Grisolia, Cesar K.

doi:10.1590/1678-4685-GMB-2017-0020

Acessibilidade / Reportar erro

Brasil

Genetics and Molecular Biology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Plant Genetics • Genet. Mol. Biol. 41 (1) • Jan-Mar 2018 • https://doi.org/10.1590/1678-4685-GMB-2017-0020 copy

Comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. and Phaseolus vulgaris L. from gene banks

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

This study aimed to assess the feasibility of comet and cytogenetic tests as tools for evaluating genomic instability in seeds of Oryza sativa L. (rice) and Phaseolus vulgaris (beans) L. from gene banks. Rice and beans were exposed to methyl methanesulfonate (MMS) as a reference DNA damaging agent. Seeds of two accessions of rice and beans were obtained from Embrapa Rice and Beans - Brazil. Seed groups were imbibed in three concentrations of MMS for three periods of time to carry out cytogenetic tests, and for one period for the comet test. At concentrations of 10 and 15 mg/L, MMS induced cytotoxic and/or mutagenic effects in the meristematic cells of roots from all the accessions of both species. In the comet test, MMS induced genotoxic effects at all the concentrations in the evaluated accessions of rice and beans, except in one accession of beans at the lowest concentration (5 mg/L). Both species showed sensitivity to MMS. The comet test can be proposed for the measurement of genomic instability in accessions of rice and beans in gene banks, as being more sensitive than the cytogenetic tests used.

Keywords:
Oryza sativa L.; Phaseolus vulgaris L.; methyl methanesulfonate; cytogenetic test; comet test

Groups	Mitotic Index	Chromosome aberrations				Micronuclei frequency
		Chromosome lagging	Fragments	Bridges	Total frequency
Control	8.42 ± 0.91	0.25 ± 0.50	-	-	0.25 ± 0.50		-
Distilled water	6.82 ± 0.68	1.00 ± 0.81	0.25 ± 0.50	-	1.25 ± 0.50	-
MMS 5 mg/L
4 h	7.42 ± 1.47	0.25 ± 0.50	-	-	0.25 ± 0.50	-
8 h	8.35 ± 0.42	1.00 ± 0.81	-	-	1.00 ± 0.81	-
24 h	7.77 ± 1.07	0.75 ± 0.95	-	0.50 ± 0.74	1.25 ± 0.95	-
MMS 10 mg/L
4 h	7.67 ± 1.17	1.75 ± 0.95	0.75 ± 0.95	-	2.50 ± 1.00	0.25 ± 0.50
8 h	7.40 ± 0.60	1.25 ± 0.95	0.75 ± 0.50	0.75 ± 0.95	2.50 ± 1.29	0.25 ± 0.50
24 h	8.40 ± 0.49	1.75 ± 1.70	0.25 ± 0.50	0.75 ± 1.50	2.75 ± 1.50^a*	-
MMS 15 mg/L
4 h	6.27 ± 0.76^a*	0.75 ± 1.50	0.50 ± 0.57	-	1.25 ± 1.25	-
8 h	6.02 ± 0.91^a**	-	0.25 ± 0.50	0.50 ± 1.00	0.75 ± 0.95	-
24 h	6.95 ± 0.62	1.50 ± 1.73	-	-	1.50 ± 1.73	-

Groups	Mitotic Index	Chromosome aberrations				Micronuclei frequency
		Chromosome lagging	Fragments	Bridges	Total frequency	Micronuclei frequency
Control	8.05 ± 1.30	-	-	-	-	-
Distilled water	6.77 ± 0.49	0.50 ± 0.74	-	-	0.50 ± 0.74	-
MMS 5 mg/L
4 h	7.42 ± 1.47	0.50 ± 0.74	-	-	0.50 ± 0.74	-
8 h	8.35 ± 0.42	0.50 ± 1.00	0.50 ± 0.74	0.50 ± 1.00	1.50 ± 1.73	-
24 h	7.77 ± 1.07	-	0.50 ± 0.74	-	0.50 ± 0.74	-
MMS 10 mg/L
4 h	7.40 ± 0.77	0.50 ± 1.00	1.25 ± 1.50	-	1.75 ± 1.25	0.25 ± 0.50
8 h	7.50 ± 0.50	1.00 ± 0.81	1.25 ± 0.95	0.50 ± 1.00	2.75 ± 1.50^a*	0.50 ± 0.57
24 h	7.17 ± 2.14	0.75 ± 1.50	2.25 ± 1.25^a;b	-	3.00 ± 1.15^a*;b	-
MMS 15 mg/L
4 h	5.77 ± 0.85^a*	0.75 ± 1.50	0.50 ± 0.57	0.25 ± 0.50	1.50 ± 2.38	-
8 h	7.00 ± 0.98	0.25 ± 0.50	0.25 ± 0.50	-	0.50 ± 0.57	-
24 h	7.85 ± 1.05	0.75 ± 0.95	0.75 ± 0.50	-	1.50 ± 1.29	-

Groups	Mitotic Index	Chromosome aberrations				Micronuclei frequency
		Chromosome lagging	Fragments	Bridges	Total frequency	Micronuclei frequency
Control	5.92 ± 1.29	-	-	-	-	-
Distilled water	5.15 ± 1.00	-	-	-	-	-
MMS 5 mg/L
4 h	5.67 ± 1.43	1.00 ± 1.15	0.75 ± 0.95	-	1.75 ± 1.70	-
8 h	5.17 ± 1.55	0.25 ± 0.50	0.50 ± 0.57	-	0.75 ± 0.95	-
24 h	3.95 ± 0.91	0.25 ± 0.50	0.75 ± 0.95	-	1.00 ± 1.15	0.50 ± 0.57
MMS 10 mg/L
4 h	4.72 ± 0.49	0.25 ± 0.50	0.50 ± 0.57	-	0.75 ± 0.50	-
8 h	4.85 ± 0.68	1.00 ± 0.00^a;b;c*	0.25 ± 0.50	-	1.25 ± 0.50^a;b	-
24 h	2.72 ± 0.47^{a*;b;c;d}	0.50 ± 0.57	-	0.25 ± 0.50	0.75 ± 0.95	0.25 ± 0.50
MMS 15 mg/L
4 h	7.87 ± 2.45	1.25 ± 0.50^{a*;b;c;e**}	-	-	1.25 ± 0.50^{a*;b;c;e**}	-
8 h	4.15 ± 0.56^c*	0.25 ± 0.50	-	-	0.25 ± 0.50	-
24 h	1.82 ± 0.90^a*;b;c***	-	-	-	-	-

Groups	Mitotic Index	Chromosome aberrations				Micronuclei frequency
		Chromosome lagging	Fragments	Bridges	Total frequency	Micronuclei frequency
Control	7.90 ± 1.60	-	-	-	-	-
Distilled water	5.97 ± 1.21	0.25 ± 0.50	-	-	0.25 ± 0.50	-
MMS 5 mg/L
4 h	8.00 ± 1.60	0.25 ± 0.50	-	0.25 ± 0.50	0.50 ± 0.57	0.25 ± 0.50
8 h	5.47 ± 2.10	0.25 ± 0.50	0.25 ± 0.50	-	0.50 ± 1.00	-
24 h	6.37 ± 2.65	0.25 ± 0.50	-	-	0.25 ± 0.50	-
MMS 10 mg/L
4 h	8.15 ± 3.37	-	-	0.25 ± 0.50	0.25 ± 0.50	-
8 h	6.67 ± 1.21	0.25 ± 0.50	-	0.25 ± 0.50	-	-
24 h	5.17 ± 1.42	1.50 ± 1.73	0.25 ± 0.50	-	1.75 ± 1.70	-
MMS 15 mg/L
4 h	8.35 ± 1.27	0.50 ± 1.00	0.25 ± 0.50	0.25 ± 0.50	1.00 ± 1.41	-
8 h	7.07 ± 1.87	0.50 ± 0.57	-	0.75 ± 1.50	1.25 ± 1.89	-
24 h	4.52 ± 0.95^a;b	1.25 ± 1.25	0.25 ± 0.50	-	1.50 ± 1.29	-

Sociedade Brasileira de Genética Rua Cap. Adelmio Norberto da Silva, 736, 14025-670 Ribeirão Preto SP Brazil, Tel.: (55 16) 3911-4130 / Fax.: (55 16) 3621-3552 - Ribeirão Preto - SP - Brazil
E-mail: editor@gmb.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Send correspondence to Cesar Grisolia. Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Darcy Ribeiro 7091-900 Brasília, DF, Brasil. E-mail: grisolia@unb.br.