Comparative cytogenetics in three <i>Melipona</i> species (Hymenoptera: Apidae) with two divergent heterochromatic patterns

Cunha, Marina Souza da; Travenzoli, Natália Martins; Ferreira, Riudo de Paiva; Cassinela, Edson Kuatelela; Silva, Henrique Barbosa da; Oliveira, Francisco Plácido Magalhães; Salomão, Tânia Maria Fernandes; Lopes, Denilce Meneses

doi:10.1590/1678-4685-GMB-2017-0330

Acessibilidade / Reportar erro

Brasil

Genetics and Molecular Biology

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Animal Genetics • Genet. Mol. Biol. 41 (4) • Oct-Dec 2018 • https://doi.org/10.1590/1678-4685-GMB-2017-0330 copy

Comparative cytogenetics in three Melipona species (Hymenoptera: Apidae) with two divergent heterochromatic patterns

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The genus Melipona is subdivided into four subgenera based on morphological characteristics, and two groups based on cytogenetic patterns. The cytogenetic information on this genus is still scarce, therefore, the goal of this study was to characterize Melipona paraensis, Melipona puncticollis, and Melipona seminigra pernigra using the following techniques: C-banding, DAPI/CMA₃ fluorochromes, and FISH with an 18S rDNA probe. Melipona paraensis (2n=18) and M. seminigra pernigra (2n=22) were classified as high heterochromatin content species (Group II). Their euchromatin is restricted to the ends of the chromosomes and is CMA₃⁺; the 18S rDNA probe marked chromosome pair number 4. Melipona puncticollis (2n=18) is a low heterochromatin content species (Group I) with chromosome pair number 1 marked with CMA₃ and 18S rDNA. Low heterochromatin content is a putative ancestral karyotype in this genus and high content is not a monophyletic trait (Melikerria presents species with both patterns). Differences concerning the karyotypic characteristics can be observed among Melipona species, revealing cytogenetic rearrangements that occurred during the evolution of this genus. Studies in other species will allow us to better understand the processes that shaped the chromatin evolution in Melipona.

Keywords:
Chromosomal evolution; DAPI/CMA₃ fluorochromes; Fluorescent in situ Hybridization (FISH); heterochromatin; Meliponini

Subgenus	Species	Chromosome Number	C-Banding	CMA₃	18S rDNA	References
Eomelipona	M. asilvai	2n = 18	Low content	2 interstitial markings	2 interstitial markings ^* * Ag-NOR data.	Rocha and Pompolo, 1998; Rocha et al., 2002; Rocha et al., 2007
	M. bicolor	2n = 18	Low content	2 interstitial markings	-	Rocha and Pompolo, 1998
	M. marginata	2n = 18	Low content	2 interstitial markings	2 interstitial markings ^* * Ag-NOR data.	Rocha and Pompolo, 1998; Maffei et al., 2001; Rocha et al., 2007
	M. puncticollis	2n = 18 (2m+14sm+2a)	Low content	2 interstitial markings	2 interstitial markings	Present study
Melikerria	M. fasciculata	2n = 18	High content	Terminal marks on all chromosomes	2 terminal markings^† † M. compressipes in the paper of Rocha et al. (2002) is indeed M. fasciculata (Tavares et al., 2017).	Rocha et al, 2002; Lopes et al., 2011
	M. quinquefasciata	2n = 18^‡	Low content	2 interstitial markings	-	Rocha et al., 2007
Melipona	M. favosa	2n = 18 (12m+4sm+2a)	-	-	-	Hoshiba, 1988
	M. mandacaia	2n = 18 (2m+14sm+2a)	Low content	2 interstitial markings	-	Rocha et al., 2003
	M. quadrifasciata	2n = 18 (4m+12sm+2a)	Low content	2 interstitial markings	-	Rocha and Pompolo, 1998
	M. subnitida	2n = 18	Low content	2 interstitial markings	-	Rocha et al., 2002; Rocha et al., 2007
Michmelia	M. capixaba	2n = 18	High content	Terminal marks on all chromosomes	-	Rocha and Pompolo, 1998; Rocha et al., 2002
	M. captiosa	2n = 18	High content	-	-	Rocha and Pompolo, 1998
	M. crinita	2n = 18	High content	Terminal marks on all chromosomes	-	Rocha et al., 2002
	M. flavolineata	2n = 18	High content	Terminal marks on all chromosomes	-	Lopes et al., 2011
	M. fuliginosa	2n = 18	High content	Terminal marks on all chromosomes	-	Lopes et al., 2011
	M. fuscopilosa	2n = 18	High content	Terminal marks on all chromosomes	-	Rocha and Pompolo, 1998; Rocha et al., 2002
	M. mondury	2n = 18	High content	Terminal marks on all chromosomes	-	Lopes et al., 2008
	M. paraensis	2n = 18	High content	Terminal marks on all chromosomes	2 terminal markings	Present study
	M. rufiventris	2n = 18^‡ ‡ B chromosomes were reported in these two species.	High content	Terminal marks on all chromosomes	-	Lopes et al., 2008
	M. scutellaris	2n = 18	High content	Terminal marks on all chromosomes	-	Rocha and Pompolo, 1998; Rocha et al., 2002
	M. seminigra merrillae	2n = 22	Low content?^§ § Reevaluated as high content. More details are given in the text.	-	-	Francini et al., 2011
	M. seminigra pernigra	2n = 22	High content	Terminal marks on all chromosomes	2 terminal markings	Present study

Sociedade Brasileira de Genética Rua Cap. Adelmio Norberto da Silva, 736, 14025-670 Ribeirão Preto SP Brazil, Tel.: (55 16) 3911-4130 / Fax.: (55 16) 3621-3552 - Ribeirão Preto - SP - Brazil
E-mail: editor@gmb.org.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Send correspondence to Denilce M. Lopes. Departamento de Biologia Geral, Universidade Federal de Viçosa, Av. P. H. Rolfs, s/n, Centro, Viçosa, 36570-900, MG, Brazil. E-mail: denilce.lopes@ufv.br.