Abstract

BOMBONATO, Juliana Rodrigues et al. Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae). Genet. Mol. Biol. [online]. 2019, vol.42, n.1, pp.87-94.  Epub Feb 21, 2019. ISSN 1415-4757.  http://dx.doi.org/10.1590/1678-4685-gmb-2017-0293.

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.

Keywords : Cactaceae; Cereus; cross-genera; SSR markers; Transferability.

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