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Revista Brasileira de Plantas Medicinais

versão impressa ISSN 1516-0572

Resumo

VICTORIO, C.P.; KUSTER, R.M.  e  LAGE, C.L.S.. Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography. Rev. bras. plantas med. [online]. 2009, vol.11, n.2, pp.147-153. ISSN 1516-0572.  http://dx.doi.org/10.1590/S1516-05722009000200006.

The species Alpinia purpurata is scarcely cited as to ethnopharmacology and phytochemistry. This study aimed to analyze bioactive compounds through high-performance liquid chromatography (HPLC). Hydroalcoholic crude extract was obtained from A. purpurata dried leaves. Folin-Ciocalteau method was used to quantify total phenols, using gallic acid as standard. The obtained result was 15.6 mg GAE g-1. The crude extract was partitioned with the solvents ethyl acetate and butanol, followed by thin-layer chromatography (TLC) and HPLC. The flavonoids kaempferol-3-O-glucuronide and rutin were detected at a higher concentration in ethyl acetate and butanolic extracts. The butanolic extract contains the highest flavonoid percentage (94.3%). A. purpurata presents important flavonoids of therapeutic use, already verified for A. zerumbet. This is the first study verifying the presence of flavonoids in A. purpurata extracts.

Palavras-chave : TLC; polyphenols; medicinal plants; rutin; Zingiberaceae.

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